摘 要：本文初步建立了HPLC(High performance liquid chromatographic method高效液相色谱法)测定高油辣椒制品中辣椒制品中两种主要辣椒素类物质的方法。样品以甲醇+正己烷进行预处理，采用反相高效液相色谱分离,紫外检测器;根据色谱峰的保留时间定性,以外标峰面积法定量辣椒素(C)、二氢辣椒素(DC)。采用色谱柱,ZORBAX SB-C18;流动相,V(甲醇)∶V(水)=65：35；UV检测波长,280 nm;辣椒素和二氢辣椒素RSD分别为5.11%,5.08%(n=5);回收率分别为89.4%、90.0%。辣椒素和二氢辣椒素在20－200ug/ml范围内线性良好。方法满足辣味水平评价要求。
关键词：辣椒；高油含量；HPLC；测定
DETERMINATION OF CAPSAICIN IN THE HIGH OIL PEPPER PRODUCTS
Abstract：A method was preliminary developed for the determination of capsaicinoids in highly oil-containing pepper productsby high performance liquid chromatography. The sample was extractedwith Methanol and Hexane. The analysis wasperformed on a C18 column (250×4.6 mm i.d.5μm) with 65∶35 Methanol-water as the mobile phase at a flow rate of 1.2 mL/min and detected at 280 nm. The determination of capsaicin(C),dihydrocapsaicin(DC) were processed by peak area andexternal standard method. The precisions of C and DC were good with the RSD of 5.11% and 5.08% espectively (n=5).The recoveries for C and DC are 89.4% and 90%respectively. The two ompounds demonstrated good linear relationship in the range of 20-200 ug/ml.
Key words：Pepper, High oil content, HPLC, Determine
目 录
摘 要
关键词
1 前言
2 材料与方法
2.1 材料
2.2 主要试剂
2.3 主要仪器设备
2.4 试验方法
2.4.1 样品提取溶剂选择性实验
2.4.2 正己烷最佳加入量的确定
2.4.3 高效液相色谱法条件选择试验
2.4.4 样品预处理
2.4.5 对照处理
2.5 标准溶液配制及绘制标准曲线.
2.6 结果计算
2.6.1 辣椒素物质总量的计算
2.6.2 二氢辣椒素物质总量的计算
3 结果与分析
3.1辣椒素提取剂的确定
3.2 正己烷加入量的确定
3.3 高效液相色谱法条件确定
3.3.1 流动相的确定
3.3.2 流速的确定
3.3.3 UV检测波波长的选择
3.3.4色谱条件
3.4 线性关系
3.5 回收率试验
3.6 精密度试验
3.7 样品测定结果
4 结论
参考文献
致 谢
